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Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
18603 42727 2007 7 PDF Available
Title
Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme
Abstract

p-Hydroxycinnamic acid (pHCA), which serves as the starting material for production of a number of industrial chemicals, can be produced by deamination of tyrosine by phenylalanine/tyrosine ammonia lyase (PAL/TAL) enzyme. In this study, we characterized the PAL/TAL enzymes from the red yeast Rhodotorula glutinis (RgTAL) and a novel thermostable PAL/TAL from the wood rotting fungus, Phanerochaete chrysosporium (PcTAL). Both enzymes were expressed at ∼50% level of total soluble proteins under the control of the araB promoter. At 25 °C and pH 9.5, the RgTAL enzyme showed kcat and Km values of 0.93 s−1 and 68 μM for tyrosine and 1.5 s−1 and 126 μM for phenylalanine, while these values, for PcTAL, at the same pH and at 35 °C, were 1.3 s−1 and 44 μM for tyrosine and 3.3 s−1 and 161 μM for phenylalanine. The purified PcTAL was thermostable and retained its full activity at 60 °C for up to 3 h, while RgTAL lost most of its activity at this temperature. Thermostability of PcTAL allowed increasing the reaction temperature which, in addition to accelerating the reaction rate, improved solubility of the tyrosine substrate, thus, allowing production of significantly higher amounts of pHCA.

Keywords
Tyrosine ammonia lyase (TAL); Phenylalanine ammonia lyase (PAL); Trans-p-hydroxycinnamic acid (p-coumarate; pHCA); Protein expression; Arabinose-inducible promoter (araB); Thermostable enzyme
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Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 1, 3 December 2007, Pages 58–64
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us