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Characterization of a new antidementia β-secretase inhibitory peptide from Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
18607 42727 2007 6 PDF Available
Title
Characterization of a new antidementia β-secretase inhibitory peptide from Saccharomyces cerevisiae
Abstract

In order to produce a potent antidementia β-secretase (BACE1) inhibitor from microorganisms, BACE1 inhibitory activities of cell-free extracts from many bacteria, yeasts and mushrooms were determined. Cell-free extracts of Saccharomyces cerevisiae K-7 exhibited the greatest BACE1 inhibitory activity of 64.2%. After purification of the BACE1 inhibitory peptides from S. cerevisiae K-7 using ultrafiltration, Sephadex G-10 column chromatography and reverse-phase HPLC, a purified BACE1 inhibitor with IC50 inhibitory activity of 72 ng (2.59 μM) was obtained with a 0.6% solid yield. The molecular mass of the purified BACE1 inhibitor was estimated to be 697 Da by LC–MS. It is a novel octapeptide with the sequence Gly-Pro-Leu-Gly-Pro-Ile-Gly-Ser, and does not show any similarity to the other BACE1 inhibitory peptide sequences. The purified BACE1 inhibitory peptide inhibited BACE1 non-competitively and was stable at 70 °C for 30 min, showing 75% of the residual BACE1 inhibitory activity.

Keywords
Antidementia; β-Secretase inhibitory peptide; Saccharomyces cerevisiae
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Characterization of a new antidementia β-secretase inhibitory peptide from Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 1, 3 December 2007, Pages 83–88
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us