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Production of an active recombinant Aspin antigen in Escherichia coli for identifying animals resistant to nematode infection

Paper ID Volume ID Publish Year Pages File Format Full-Text
18613 42728 2006 8 PDF Available
Title
Production of an active recombinant Aspin antigen in Escherichia coli for identifying animals resistant to nematode infection
Abstract

Production of recombinant Aspin, a aspartyl protease inhibitor homologue produced by the parasitic nematode Trichostrongylus colubriformis, in Escherichia coli is reported. Culture conditions were investigated for maximizing the production of Aspin in soluble bioactive form as opposed to inclusion body. High growth and expression rates caused preferential production of inclusion bodies. In fed-batch fermentations, controlling expression at low values by decreasing the bioreactor temperature, dissolved oxygen level and concentration of nutrients, all proved effective in enhancing the production of soluble Aspin. A high volumetric titre of 220 mg/L Aspin was attained in batch fermentations induced with 2 g/L l-arabinose with the postinduction temperature reduced to 25 °C from 37 °C. The pH and dissolved oxygen levels were not controlled, as acidic final pH values and low dissolved oxygen levels favored production of soluble Aspin.

Keywords
Trichostrongylus colubriformis; Escherichia coli; Nematode infection; Aspin; Diagnostic immunoassay
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Production of an active recombinant Aspin antigen in Escherichia coli for identifying animals resistant to nematode infection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issue 5, 2 March 2006, Pages 591–598
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us