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Incorporation of pectinmethylesterase in apple tissue either by soaking or by vacuum-impregnation

Paper ID Volume ID Publish Year Pages File Format Full-Text
18615 42728 2006 7 PDF Available
Title
Incorporation of pectinmethylesterase in apple tissue either by soaking or by vacuum-impregnation
Abstract

The penetration of an exogenous Aspergillus niger pectinmethylesterase added by soaking or by vacuum-impregnation in apple cubes was examined by three different methods. The enzyme activity was first quantitatively assayed in different concentric zones of 1.5 cm apple cubes. Exogenous enzyme activity was distributed homogeneously after vacuum-impregnation, while the penetration of the enzyme was limited to the superficial zone (<2 mm depth) after soaking for 1 h. The second and third methods consisted in blotting the inner surface of cubes on a nitrocellulose membrane subsequently revealed with antibodies directed against the exogenous enzyme and in observing by fluorescence microscopy apple cubes soaked or vacuum-impregnated with fluorescein-labelled enzyme, respectively. The quantitative results were confirmed by these qualitative visual methods at a macroscopic and at a microscopic scale, respectively. Vacuum-impregnation can thus be used to add this exogenous enzyme to fruit pieces more rapidly and more homogeneously than soaking in order to improve the firmness of thermally treated fruits.

Keywords
Infusion; Vacuum-impregnation; Fruits; Enzyme; Pectinmethylesterase
First Page Preview
Incorporation of pectinmethylesterase in apple tissue either by soaking or by vacuum-impregnation
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issue 5, 2 March 2006, Pages 610–616
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering