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Chitin in a dual role as substrate for Streptomyces griseus and as adsorbent for streptomycin produced during fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
18621 42728 2006 8 PDF Available
Title
Chitin in a dual role as substrate for Streptomyces griseus and as adsorbent for streptomycin produced during fermentation
Abstract

Streptomycin production from chitin by Streptomyces griseus was compared using two different types of bioreactor. The first was equipped with a combined U-shaped paddle and Rushton impeller. Also employed was a bioreactor of novel design in which the chitin was contained in a wire mesh basket that was totally submerged in a liquid salts medium. During operation the chitin was gently fluidised by air admitted into the basket. Fermentation was continued in both bioreactors until maximum antibiotic titres were achieved whereupon operation was interrupted to allow the streptomycin adsorbed to the chitin substrate to be extracted into pH 3.0 buffer before continuing fermentation of the same batch of chitin a second time. At a chitin concentration of 10% (w/v) the highest streptomycin yields (ca. 5.5 mg/l) were obtained using the stirred bioreactor, however, growth occurred more rapidly in the vertical basket bioreactor.

Keywords
Chitin; Streptomyces; Streptomycin; Stirred bioreactors; Vertical basket bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issue 5, 2 March 2006, Pages 657–664
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us