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Expression of a heterologous laccase by Aspergillus niger cultured by solid-state and submerged fermentations

Paper ID Volume ID Publish Year Pages File Format Full-Text
18622 42728 2006 5 PDF Available
Title
Expression of a heterologous laccase by Aspergillus niger cultured by solid-state and submerged fermentations
Abstract

Laccase gene IV from Trametes versicolor was inserted by REMI in Aspergillus niger C28B25. One selected transformant (C28eco3) was subjected to a second round of transformation and a new transformant was isolated (C28eco3–13). Laccase production was compared for these strains in submerged fermentation (SmF) and solid-state fermentation (SSF) using glucose or maltodextrin at different concentrations (10, 50 and 100 g/L). Enzymes yields were higher with glucose as a carbon source. However, repression of laccase was evident for SmF after increasing glucose from 10 to 50 g/L. In contrast, in SSF glucose at 50 g/L allowed the production of the highest laccase activity for strain C28eco3 (362 U/L) and C28eco3–13 (592 U/L). Advantages of SSF as an expression and production system over SmF are also discussed.

Keywords
Aspergillus niger; Laccase; Heterologous protein; Solid-state fermentation; Catabolic repression
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Expression of a heterologous laccase by Aspergillus niger cultured by solid-state and submerged fermentations
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issue 5, 2 March 2006, Pages 665–669
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us