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Comparison of the l-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
18642 42731 2007 8 PDF Available
Title
Comparison of the l-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells
Abstract

Production of l-malic acid from fumaric acid was performed by the action of the fumarase isolated from porcine heart and the fumarase in permeabilized baker's yeast cells. For each biocatalyst kinetic parameters were determined by measuring the initial reaction rate, and confirmed in the batch experiments. It was found that this reaction is inhibited by the product. Equilibrium conversion of about 80% was achieved in a batch mode by both biocatalysts. No by-product was detected during the l-malic production by permeabilized yeast cell. It was calculated by using the proposed model and experimentally verifies that 10 mg isolated fumarase gives the identical productivity in this biotransformation as 94.4 g of baker's yeast.

Keywords
l-Malic acid; Fumarase; Baker's yeast; Permeabilization; Modelling
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Comparison of the l-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 5, 1 October 2007, Pages 605–612
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us