fulltext.study @t Gmail

Optimizing the production of cutinase by Fusarium oxysporum using response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
18643 42731 2007 7 PDF Available
Title
Optimizing the production of cutinase by Fusarium oxysporum using response surface methodology
Abstract

In the present study, the authors measured the production of cutinase by Fusarium oxysporum in the presence of several carbon and nitrogen sources (glycides, fatty acids and oils, and several organic and inorganic nitrogen sources), trying to find a cost-effective substitute for cutin in the culture medium as an inducer of cutinase production. The results were evaluated by the Tukey test, and flaxseed oil was found to give the best results as a cutinase inducer. Optimization of the growth medium was carried out using response surface methodology, aiming at optimizing the concentrations of the key components. The experimental results were fitted to a second-order polynomial model at a 95% level of significance (P < 0.05). The greatest cutinolytic activity was obtained in a liquid mineral medium supplemented with flaxseed oil, showing an increase in enzymatic activity from 11 to 22.68 μMol/min mL after 48 h of fermentation. The results support the use of flaxseed oil as a substitute for cutin, which is difficult and expensive to obtain, for the production of cutinase in a larger scale.

Keywords
Cutinase; Fusarium oxysporum; Media optimization; Fractional factorial design; Central composite design
First Page Preview
Optimizing the production of cutinase by Fusarium oxysporum using response surface methodology
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 5, 1 October 2007, Pages 613–619
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us