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Elimination of glucose in egg white using immobilized glucose oxidase

Paper ID Volume ID Publish Year Pages File Format Full-Text
18657 42732 2006 6 PDF Available
Title
Elimination of glucose in egg white using immobilized glucose oxidase
Abstract

A commercial glucose oxidase containing catalase (NOVOZYM 771) was immobilized on Amberlite UP 900 anion exchange resin using adsorption and subsequent cross-linking with glutaraldehyde. The maximum protein loading was 33.0 mg g−1 dry support. The activity of the product was 10.2 units g−1 dry solid. The immobilized enzyme was used for the elimination of glucose from egg white in a pilot-scale three-stage fluidized-bed bioreactor with mechanical mixing, equipped with large porous surface for bubble-free transport of oxygen as co-substrate. The biocatalyst packing (10.9 dm3) was uniformly distributed between the stages. The best productivity was achieved using oxygen pressure of 1.5 bar and 2 h residence time at 25 °C.

Keywords
Egg white processing; Glucose elimination; Glucose oxidase immobilized; Fluidized-bed bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 5, 4 September 2006, Pages 1002–1007
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us