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Influence of the hollow-fibre membrane on the stability of β-galactosidase and on lactose hydrolysis: Kinetic models including adsorption of the enzyme onto the membrane

Paper ID Volume ID Publish Year Pages File Format Full-Text
18658 42732 2006 8 PDF Available
Title
Influence of the hollow-fibre membrane on the stability of β-galactosidase and on lactose hydrolysis: Kinetic models including adsorption of the enzyme onto the membrane
Abstract

An adsorption-kinetic model has been proposed to explain β-galactosidase stability in a recirculation hollow-fibre bioreactor at different temperature. The kinetic constants of the model and its dependence on temperature have been evaluated.In addition, a study has been made of the influence of lactose and enzyme concentration on lactose hydrolysis with hollow-fibre modules made of Cuprophan® and Polysulfone®. The membrane surface areas assayed were between 1 and 1.95 m2. The experimental results have been satisfactorily explained with the model proposed in a previous work. This model included the competitive inhibition of the enzyme by galactose and the enzymatic adsorption onto the membrane. The adsorption constants have been calculated, verifying the applicability of the model in the modules used. Within the experimental range analysed, the hollow-fibre module made of Polysulfone® provided the least adsorption of the enzyme and therefore was the most suitable for this enzyme and reaction.

Keywords
Kinetic model; Enzymatic stability; Membrane adsorption; Hollow-fibre bioreactor; Lactose hydrolysis
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Influence of the hollow-fibre membrane on the stability of β-galactosidase and on lactose hydrolysis: Kinetic models including adsorption of the enzyme onto the membrane
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 5, 4 September 2006, Pages 1008–1015
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us