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Production by Clonostachys compactiuscula of a lovastatin esterase that converts lovastatin to monacolin J

Paper ID Volume ID Publish Year Pages File Format Full-Text
18664 42732 2006 9 PDF Available
Title
Production by Clonostachys compactiuscula of a lovastatin esterase that converts lovastatin to monacolin J
Abstract

The conversion of monacolin K (lovastatin) to monacolin J, a core structure in the synthesis of other statins, was achieved using the fungus Clonostachys compactiuscula and optimized with response surface methodology. To study the proposed second-order polynomial model, a central composite experimental design with multiple linear regression was used to estimate the model coefficients of five selected factors believed to influence the conversion process. The experimental results indicated that the optimal conditions for growth of C. compactiuscula mycelium were as follows: 2.0 g glucose/L, initial pH of the medium 8.5, and incubation of the mycelium for 4 days. These conditions yielded an optimal concentration of the substrate lovastatin of 1 mg/mL, and a conversion time of 15 h. A lovastatin esterase was isolated and purified from the mycelium of C. compactiuscula by ammonium sulfate precipitation, size-exclusion chromatography, and ion-exchange chromatography. Following SDS-PAGE, the purified enzyme appeared as a single band with an apparent molecular mass of 28 kDa. The converted product, monacolin J, was isolated and purified, and its structure was analyzed by NMR.

Keywords
Clonostachys compactiuscula; Hypercholesterolemia; Lovastatin; Monacolin esterase; Response surface methodology
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Production by Clonostachys compactiuscula of a lovastatin esterase that converts lovastatin to monacolin J
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 5, 4 September 2006, Pages 1051–1059
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us