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Production of valienamine by a newly isolated strain: Stenotrophomonas maltrophilia

Paper ID Volume ID Publish Year Pages File Format Full-Text
18665 42732 2006 6 PDF Available
Title
Production of valienamine by a newly isolated strain: Stenotrophomonas maltrophilia
Abstract

Valienamine, an aminocyclitol, is an important medicinal intermediate with broad use in the synthesis of some stronger α-glucosidase inhibitors. The bacterial strain Stenotrophomonas maltrophilia CCTCC M 204024 was recently isolated in our laboratory for production of valienamine from validamycin A. In this study, conditions were varied to investigate valienamine production using this strain. We found the optimized conditions included (1) use of validamycin A as the sole carbon source (initial concentration of 15 g L−1), (2) use of (NH4)2SO4 as the sole nitrogen source, (3) pH 7.0, (4) temperature range between 28 and 37 °C, and (5) kLa of 133 h−1. Under these optimal conditions, an average of 1.15 g L−1 valienamine was obtained after fermentation for 6 d. Fermentation in a 15-L mechanically stirred tank bioreactor containing 10 L of culture medium was also done to observe valienamine production. The concentration in culture broth reached 1.09 g L−1 after 6 d of fermentation. It is concluded that degradation of validamycin A by S. maltrophilia is a promising method to achieve an effective way to production of valienamine.

Keywords
Enzyme inhibitor; Stenotrophomonas maltrophilia; Validamycin A; Valienamine
First Page Preview
Production of valienamine by a newly isolated strain: Stenotrophomonas maltrophilia
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 5, 4 September 2006, Pages 1060–1065
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering