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Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation

Paper ID Volume ID Publish Year Pages File Format Full-Text
18671 42732 2006 5 PDF Available
Title
Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
Abstract

Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate.

Keywords
Carica papaya latex; Papain; Aqueous two-phase extraction; Two-step salt precipitation
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Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 5, 4 September 2006, Pages 1103–1107
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us