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In situ electrochemical enzyme immunoassay on a microchip with surface-functionalized poly(dimethylsiloxane) channel

Paper ID Volume ID Publish Year Pages File Format Full-Text
18674 42732 2006 6 PDF Available
Title
In situ electrochemical enzyme immunoassay on a microchip with surface-functionalized poly(dimethylsiloxane) channel
Abstract

This paper reports a rapid microchip-based electrochemical enzyme immunoassay with surface-functionalized poly(dimethylsiloxane) (PDMS) channel. The microchip fabricated on a glass substrate by photolithography consists of a three-electrode system for electrochemical detection and is assembled with the antibody-immobilized PDMS channel by plasma treatment. For effective immobilization of target antibodies, the internal surface of the PDMS channel is chemically modified into the silane monolayer containing poly(ethyleneglycol) (PEG) groups, which minimize non-specific binding. Due to low height (50 μm) of the channel dimensions, the microchip provides a rapid immunoassay by reducing the mass transfer resistance between electrodes and immobilized antibodies, and it takes about 8 min for total analysis time including sampling, reaction and washing step. In this study, sandwich immunoassays for mouse IgG are performed as a model system and result in a low detection limit of 485 pg mL−1 with 95% confidence level. All immunoassay procedures for liquid handling such as sampling, reaction and washing are carried out using conventional syringe pumps, preserving an internal channel volume of 0.7 μL constant. Such an on-chip integration of immunological and enzymatic reactions, amperometric detection, and microchip operations allows performing immunoassays more rapidly, easily, and economically.

Keywords
AP, alkaline phosphatase; BSA, bovine serum albumin; EDC, 1-ethyl-3-(dimethylamino)-propylcarbodiimide; HEPES, N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid]; IgG, immunoglobulin G; MES, 1-(N-morpholino)ethanesulfonic acid; PAP, p-aminophenol; PA
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In situ electrochemical enzyme immunoassay on a microchip with surface-functionalized poly(dimethylsiloxane) channel
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 5, 4 September 2006, Pages 1122–1127
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us