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Optimization of nutrient feed concentration and addition time for production of poly(β-hydroxybutyrate)

Paper ID Volume ID Publish Year Pages File Format Full-Text
18677 42732 2006 7 PDF Available
Title
Optimization of nutrient feed concentration and addition time for production of poly(β-hydroxybutyrate)
Abstract

PHB is an intracellular storage compound produced by many organisms under conditions of nutrient limitation (N, P, etc.) when carbon source is present in excess. Model-based fed-batch cultivation is one of the techniques to induce desired nutrient limitations intelligently for over production of such partially growth-associated polymeric products. In the present study, batch kinetics of PHB synthesis by Wautersia eutropha was established in a bioreactor. Batch kinetics featured a biomass concentration of 19.68 g/l with a PHB content of 10.89 g/l in 60 h. A mathematical model was then developed, which adequately described the batch kinetics. The model equations were extrapolated to fed-batch cultivation by incorporating the dilution terms due to fresh nutrient (carbon and nitrogen) feeding. Model-based fed-batch fermentation was conducted by feeding nitrogen (7 g/l) from 20–30 h at the rate of 70 ml/h and fructose (300 g/l) from 24–34 h at the rate of 80 ml/h constantly which yielded 32 g/l biomass containing 14 g/l PHB in 50 h. The productivity was 1.5-times greater than that obtained in batch increasing from 0.18 to 0.28 g/l-h. The model proved to be highly instrumental in the design of suitable fed-batch cultivation.

Keywords
PHB, poly(β-hydroxybutyrate); SSWR, sum of squares of weighed residuesWautersia eutropha; Mathematical model; PHB; Model-based fed-batch cultivation
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Optimization of nutrient feed concentration and addition time for production of poly(β-hydroxybutyrate)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 5, 4 September 2006, Pages 1145–1151
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us