fulltext.study @t Gmail

Novel method of enzymes stabilization on crosslinked thermosensitive carriers

Paper ID Volume ID Publish Year Pages File Format Full-Text
18692 42735 2006 5 PDF Available
Title
Novel method of enzymes stabilization on crosslinked thermosensitive carriers
Abstract

In this paper an immobilization of invertase on thermosensitive copolymers of N-isopropylacrylamide and 2-hydroxyethyl methacrylate or glycidyl methacrylate modified by aminolysis is evaluated. The method is based on the swelling properties of stimuli-sensitive polymers, which work like a pump that sucks up the enzyme on cooling and then on subsequent crosslinking of the enzyme. The attention was focused on the properties of the carrier–enzyme systems, particularly on the effect of crosslinking on their stability. Activity of TG8-NH2 carrier was very low and independent on concentration of glutaraldehyde used, but carriers TH8 and TH8-NH2 were more active, especially when 1.0 and 2.5 vol.% of glutaraldehyde were used. It was also observed, that preparations crosslinked by glutaraldehyde were more stable than preparations without crosslinking agent.

Keywords
Immobilization; Invertase; Thermosensitive carriers
First Page Preview
Novel method of enzymes stabilization on crosslinked thermosensitive carriers
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issue 7, 2 May 2006, Pages 921–925
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us