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Ester production in E. coli and C. acetobutylicum

Paper ID Volume ID Publish Year Pages File Format Full-Text
18695 42735 2006 7 PDF Available
Title
Ester production in E. coli and C. acetobutylicum
Abstract

Genetic engineering has improved the product yield of a variety of compounds by overexpressing, inactivating, or introducing new genes in microbial systems. The production of flavor-enhancing ester compounds is an emerging area of heterologous gene expression for desired product yield in Escherichia coli. Isoamyl acetate, butyl acetate, ethyl acetate, and butyl butyrate are reported here to be produced by expressing Saccharomyces cerevisiae genes ATF1 or ATF2 and the strawberry gene SAAT in E. coli when the appropriate substrates are provided. Increasing the concentration of alcohol added to the reaction generally resulted in increased ester production. ATF1 expression was found to produce more isoamyl acetate and butyl acetate than ATF2 expression or SAAT expression in the strains and culture conditions examined. Additionally, SAAT expression resulted in greater isoamyl acetate and butyl acetate production than ATF2 expression. Butyl butyrate is produced by cell-free extracts of E. coli harboring SAAT but not ATF1 or ATF2.

Keywords
Clostridium acetobutylicum; Ester; Isoamyl; Isoamyl acetate; Butyl acetate; Butyl butyrate; Alcohol acetyltransterase
First Page Preview
Ester production in E. coli and C. acetobutylicum
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issue 7, 2 May 2006, Pages 937–943
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering