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Ethanol tolerance, yield of melanin, swarming motility and growth are correlated with the expression levels of aiiA gene in Bacillus thuringiensis

Paper ID Volume ID Publish Year Pages File Format Full-Text
18699 42735 2006 8 PDF Available
Title
Ethanol tolerance, yield of melanin, swarming motility and growth are correlated with the expression levels of aiiA gene in Bacillus thuringiensis
Abstract

To investigate the role of aiiA gene in Bacillus thuringiensis, B. thuringiensis strain BMB171 was selected and three derivatives were constructed, such that (1) aiiA was knocked out (aiiA-KO), (2) aiiA-KO was reversed (aiiA-Rev) and (3) the expression of aiiA was elevated (aiiA-Elevated). First, we found that mutation of aiiA increased the ethanol tolerance in B. thuringiensis. Omitting ethanol from the medium resulted in a decreased generation time for aiiA-Elevated. In contrast, aiiA-KO had the highest ethanol tolerance when the medium contained 2 or 4% ethanol. Second, we found that more melanin was produced at 37 °C when aiiA expression was elevated. Third, we found inhibition of swarming motility in the aiiA-Elevated group. Finally, there was delayed sporulation in Luria–Bertani, ICPM, casein and ammonium sulfate medium in the aiiA-Elevated group. The conclusion was that ethanol tolerance, yield of melanin, swarming motility and growth are correlated with the expression levels of aiiA gene in B. thuringiensis.

Keywords
B. thuringiensis; AHL lactonase; Melanin; Ethanol tolerance; Growth; Swarming motility
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Ethanol tolerance, yield of melanin, swarming motility and growth are correlated with the expression levels of aiiA gene in Bacillus thuringiensis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issue 7, 2 May 2006, Pages 967–974
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us