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Immobilization of glucoamylase and trypsin on crosslinked thermosensitive carriers

Paper ID Volume ID Publish Year Pages File Format Full-Text
18710 42738 2007 8 PDF Available
Title
Immobilization of glucoamylase and trypsin on crosslinked thermosensitive carriers
Abstract

In this paper the immobilization of glucoamylase and trypsin on thermosensitive copolymers of N-isopropylacrylamide and 2-hydroxyethyl methacrylate or glycidyl methacrylate modified by amination is evaluated. The method is based on the swelling properties of these stimuli-sensitive polymers. On cooling the polymers expand and work like a pump that sucks up the enzyme adsorbed onto their surface and then the enzyme is cross-linked with glutaraldehyde, thus forming an interpenetrated polymer network. The attention was focused on the properties of the carrier–enzyme systems, particularly on the effect of cross-linking on their storage stability. The data obtained for unmodified thermosensitive carriers and carriers hydrophilised with ethylenediamine were compared. In the case of both enzymes, TH8-NH2 carrier is the most suitable, as the highest activity of immobilized enzymes is retained. Low molecular weight substrates are preferred in both cases.

Keywords
Immobilization; Glucoamylase; Trypsin; Thermosensitive carriers
First Page Preview
Immobilization of glucoamylase and trypsin on crosslinked thermosensitive carriers
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 3, 2 August 2007, Pages 197–204
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering