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Isolation of glycolonitrile-hydrolyzing microorganism based on colorimetric reaction

Paper ID Volume ID Publish Year Pages File Format Full-Text
18716 42738 2007 6 PDF Available
Title
Isolation of glycolonitrile-hydrolyzing microorganism based on colorimetric reaction
Abstract

To isolate glycolonitrile-hydrolyzing microorganisms, a simple and rapid high-throughput screening method based on a colorimetric reaction of glycolic acid with β-naphthol in sulfuric acid solution was developed. Four strains able to convert glycolonitrile to glycolic acid were isolated from soil samples using this screening method, among which Rhodococcus sp. ZJUT-N595 displayed the highest hydrolytic activity. Resting cells of Rhodococcus sp. ZJUT-N595 could also biotransform acrylonitrile, racemic 2,2-dimethylcyclopropanecarbonitrile to corresponding amides as well as carboxylic acids, and racemic 2,2-dimethylcyclopropanecarboxamide to (S)-2,2-dimethylcyclopropanecarboxlic acid (e.e. > 90%), which demonstrated that this strain contained both intracellular nitrile hydratase and amidase activities. Batch cultivation showed that the optimal growth temperature and initial medium pH for Rhodococcus sp. ZJUT-N595 glycolonitrile-hydrolyzing activity were 30 °C and pH 8, respectively. The preferred carbon and nitrogen source and ideal inducer were mannitol, a composite of peptone plus yeast extract and caprolactam.

Keywords
High-throughput screening; Glycolic acid; β-Naphthol; Glycolonitrile-hydrolyzing; Biotransformation
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Isolation of glycolonitrile-hydrolyzing microorganism based on colorimetric reaction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 3, 2 August 2007, Pages 244–249
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us