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Production of O-acetylated oligouronides by depolymerization of a natural highly acetylated anionic bacterial polysaccharide

Paper ID Volume ID Publish Year Pages File Format Full-Text
18717 42738 2007 8 PDF Available
Title
Production of O-acetylated oligouronides by depolymerization of a natural highly acetylated anionic bacterial polysaccharide
Abstract

The enzymatic depolymerization of a nascent high O-acetylated glucuronan has been studied to envisage and evaluate a possible O-acetylated oligouronides production and purification process. This paper investigates the significance of the operating parameters for the large production of anionic O-acetylated-oligosaccharides with degree of polymerization up to 10 produced by degradation of bacterial O-acetylated-β-(1,4)-d-polyglucuronic using a glucuronan lyase (GL2) from Trichoderma sp. During the glucuronan biosynthesis none oligosaccharide was obtained even if the glucuronan lyase was added before or at the end of the glucuronan biosynthesis. In such conditions, just O-acetylated MMWs and LMWs with dp > 10 were recovered. However, polymeric families with molecular weight lower than 100 kDa and partially purified using tangential ultrafiltration (UFT) were totally degraded into oligosaccharides. All these oligouronides were isolated, fractionated by low-pressure size liquid chromatography and characterized by NMR spectroscopy to apprehend their O-acetyl distribution and degree of polymerization. This study allows the production of O-acetylated oligoglucuronan in order to envisage new therapeutic applications fields such as the realization of biological tests among animals and vegetals.

Keywords
Acetyl; Anionic oligosaccharides; Glucuronan; Glucuronan lyase; Large production
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Production of O-acetylated oligouronides by depolymerization of a natural highly acetylated anionic bacterial polysaccharide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 3, 2 August 2007, Pages 250–257
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us