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Purification of a new manganese peroxidase of the white-rot fungus Schizophyllum sp. F17, and decolorization of azo dyes by the enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
18718 42738 2007 7 PDF Available
Title
Purification of a new manganese peroxidase of the white-rot fungus Schizophyllum sp. F17, and decolorization of azo dyes by the enzyme
Abstract

The manganese peroxidase (MnP) was isolated and purified from Schizophyllum sp. F17 on pinewood solid-state cultures. Fractionation of MnP was performed by Sephadex G-75 gel filtration chromatography followed by DEAE-cellulose anion exchange chromatography. This purification attained 3.8% activity yield with a purification factor of 9.2. According to data on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the molecular weight of the enzyme was 48.7 kDa. The enzyme was a glycoprotein with 18% of its weight in carbohydrates. The optimum pH and temperature of purified MnP for oxidation of DMP were 6.8 and 35 °C, respectively. This enzyme was stable in the pH range 4.0–7.0, at 25 °C for 1 h incubation period. The effects of possible inhibitors and activators of enzyme activity were investigated. The Km values of MnP for Mn2+, H2O2, guaiacol, ABTS and DMP were 35.2, 6.7, 13.1, 47.8 and 122.9 μM at pH 4.5, respectively. The azo dyes such as Congo Red, Orange G and Orange IV were efficiently decolorized using the MnP purified.

Keywords
Schizophyllum sp. F17; Manganese peroxidase; Purification; Azo dyes; Decolorization
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Purification of a new manganese peroxidase of the white-rot fungus Schizophyllum sp. F17, and decolorization of azo dyes by the enzyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 3, 2 August 2007, Pages 258–264
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us