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Immobilization of the recombinant xylanase B (XynB) from the hyperthermophilic Thermotoga maritima on metal-chelate Eupergit C 250L

Paper ID Volume ID Publish Year Pages File Format Full-Text
18721 42738 2007 8 PDF Available
Title
Immobilization of the recombinant xylanase B (XynB) from the hyperthermophilic Thermotoga maritima on metal-chelate Eupergit C 250L
Abstract

Immobilization of XynB from the hyperthermophilic Thermotoga maritima on nickel-chelate IDA (iminodiacetic acid disodium salt monohydrate)-Eupergit C 250L (Ni-E, nickel-chelate Eupergit C 250L) resulted in high immobilization yield (76.3%), catalytic efficiency (77.9%) and bond protein (96.6%). Thermostability of Ni-E XynB was significantly improved by 1 mol l−1 phosphate buffer (pH 7.0) treatment. Chemical surface modifications were followed by FT-NIR (Fourier transform near infrared) spectroscopy, strongly indicated that a chemical reaction between the amines or some other groups and epoxy groups took place in Eupergit C 250L during immobilization. Characterization of the immobilized XynB was further evaluated. The optimum pH was not affected by immobilization, but the optimum pH range of the immobilized XynB was pH 4.6–6.6 while that of the free XynB was pH 5.6–7.5. The free XynB had an optimum temperature of 90 °C, whereas that of the immobilized XynB was shifted to 100 °C. Immobilization increased both pH stability and thermostability when compared with the free enzyme. Autohydrolysis liquor of corncob (ALC) was used as the substrate for enzymatic hydrolysis by the immobilized XynB in a packed-bed enzyme reactor. The major hydrolysis product is xylobiose. The immobilization procedure developed provides a promising solution for application of xylanases in continuous hydrolysis of ALC.

Keywords
CAPS, (cyclohexylamino)-1-propanesulphonic acid; ALC, autohydrolysis liquor of corncob; Co-E; Cu-E; Ni-E and Zn-E, metal (Co2+, Cu2+, Ni2+ and Zn2+)-chelate Eupergit C 250L; DP, degree polymerization; IDA, iminodiacetic acid disodium salt monohydrate; Ni-
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Immobilization of the recombinant xylanase B (XynB) from the hyperthermophilic Thermotoga maritima on metal-chelate Eupergit C 250L
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 3, 2 August 2007, Pages 278–285
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us