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Microbial resolution of dl-homoserine for the production of d-homoserine using a novel isolate, Arthrobacter nicotinovorans strain 2-3

Paper ID Volume ID Publish Year Pages File Format Full-Text
18728 42738 2007 4 PDF Available
Title
Microbial resolution of dl-homoserine for the production of d-homoserine using a novel isolate, Arthrobacter nicotinovorans strain 2-3
Abstract

Microbial optical resolution was investigated for the practical production of d-homoserine from dl-homoserine. A bacterial strain, 2-3, that enantioselectively degrades the l-enantiomer from dl-homoserine was isolated and identified as Arthrobacter nicotinovorans. Strain 2-3 grew on medium containing 5% (w/v) dl-homoserine hydrobromide (275 mM) as the sole carbon and nitrogen source and enantioselectively exhausted the l-enantiomer in 48 h. The d-enantiomer was recovered from the culture broth with an enantiomeric excess of >99.9%. In addition, washed cells of the strain 2-3 were able to perform complete optical resolution at an initial dl-homoserine hydrobromide concentration of up to 510 mM, with a velocity of 0.28 mmol (g cell dry weight)−1 h−1.

Keywords
d-Homoserine; Microbial optical resolution; Arthrobacter nicotinovorans
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Microbial resolution of dl-homoserine for the production of d-homoserine using a novel isolate, Arthrobacter nicotinovorans strain 2-3
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 3, 2 August 2007, Pages 318–321
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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