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Efficacy of mediators for enhancing the laccase-catalyzed oxidation of aqueous phenol

Paper ID Volume ID Publish Year Pages File Format Full-Text
18734 42738 2007 9 PDF Available
Title
Efficacy of mediators for enhancing the laccase-catalyzed oxidation of aqueous phenol
Abstract

Laccase from Trametes versicolor was characterized in terms of its catalytic stability and reactivity towards phenol as a model substrate in the absence or presence of four common redox mediators. Laccase was inactivated when incubated in the presence of mediators including 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS), violuric acid (VA), 1-hydroxybenzotriazole (HBT) and 2,2′,6,6′-tetramethylpiperidine-N-oxyl (TEMPO) at pH 6 and 25 °C. Monophasic and biphasic exponential decay expressions were successfully used to model laccase inactivation under these conditions. Phenol transformation in the absence of mediators was optimum at pH 6. The addition of HBT or VA moderately enhanced phenol transformation between pH 3 and 5. TEMPO and ABTS significantly enhanced both the extent and rates of phenol transformation, particularly at pH 6. Over the course of a reaction, some mediator species gradually transformed into compounds that were no longer active as mediators. In general, the use of mediators enhanced phenol transformation; however, in certain instances their practical application may be limited since they contribute to laccase inactivation and considerable quantities are required.

Keywords
Laccase; Mediator; 1-Hydroxybenzotriazole; Violuric acid; TEMPO; ABTS; Inactivation; Phenol; Oxidation; Transformation; Enzyme stability; Exponential decay
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Efficacy of mediators for enhancing the laccase-catalyzed oxidation of aqueous phenol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 3, 2 August 2007, Pages 353–361
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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