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Potential of a Pycnoporus sanguineus laccase in bioremediation of wastewater and kinetic activation in the presence of an anthraquinonic acid dye

Paper ID Volume ID Publish Year Pages File Format Full-Text
18736 42738 2007 9 PDF Available
Title
Potential of a Pycnoporus sanguineus laccase in bioremediation of wastewater and kinetic activation in the presence of an anthraquinonic acid dye
Abstract

In the frame of the development of a bioprocess using the competences of White Rot Fungi to decolourise and detoxify dye contaminated effluents, laccases were produced by the strain Pycnoporus sanguineus MUCL 41582 in a malt extract medium. Two isoenzymes were detected among which LAC-1 was concentrated. A survey of the composition of industrial effluents showed that wastewaters from textile industry usually contain high concentrations of Na2SO4 or NaCl. Regarding the activity profile of the biocatalyst against pH, salts, temperature and target substrates, LAC-1 appears to be a good candidate for application in acid dye bath treatments. Studying the model anthraquinonic dye ABu62 decolourisation, we proved that this dye was a good substrate for LAC-1. Furthermore, unusual kinetic behaviour was observed suggesting that LAC-1 was activated in presence of ABu62. On the contrary, a classical Michaelis-Menten behaviour was observed for the oxidation of ABTS and LAC-1 showed a high affinity for this substrate as compared to data available for other laccases. To our knowledge, this is the first report showing this atypical behaviour of a laccase in the presence of dyes.

Keywords
ABTS, 2′,2-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic acid); ABu62Acid Blue, 62 (anthraquinonic acid dye); AOr7Acid Orange, 7; AYe36Acid Yellow, 36; BCCM™/MUCL, Belgian coordinated collections of microorganisms/mycothèque de l’Université catholique de
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Potential of a Pycnoporus sanguineus laccase in bioremediation of wastewater and kinetic activation in the presence of an anthraquinonic acid dye
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 3, 2 August 2007, Pages 368–376
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us