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Inhibition of Clostridium histolyticum supernatant cytotoxic activity by protease inhibitors

Paper ID Volume ID Publish Year Pages File Format Full-Text
18746 42741 2006 4 PDF Available
Title
Inhibition of Clostridium histolyticum supernatant cytotoxic activity by protease inhibitors
Abstract

Clostridium histolyticum supernatant, thanks to intrinsic proteases, exerts cytotoxic effect on cells and tissues. In our study we compared the ability of five different protease inhibitors to block cytotoxic activity of C. histolyticum supertanant: aprotinin, leupeptin, phenylmethylsulphonyl fluoride (PMSF), l-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl (TLCK) and chymostatin. Inhibitors of serine proteases nearly completely blocked cytotoxic effect, especially in lower supernatant concentrations. At the same time TLCK, known to irreversibly block clostripain obtained from C. histolyticum, blocked enzymatic activity of clostripain, but not cytotoxic effect of the supernatant. We conclude that the remaining cytotoxicity must be the effect of the activity of another factor that is contained in the supernatant and that can be isolated and tested in the presence of TLCK.

Keywords
Clostridium histolyticum; Cytotoxicity; Protease inhibitors
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Inhibition of Clostridium histolyticum supernatant cytotoxic activity by protease inhibitors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 1, 1 June 2006, Pages 28–31
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us