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Fed batch culture with declining specific growth rate for high-yielding production of a plasmid containing a gene therapy sequence in Escherichia coli DH1

Paper ID Volume ID Publish Year Pages File Format Full-Text
18749 42741 2006 4 PDF Available
Title
Fed batch culture with declining specific growth rate for high-yielding production of a plasmid containing a gene therapy sequence in Escherichia coli DH1
Abstract

Using a linear fed batch culture system, we obtained a yield of 296 mgDNA L−1 of a non-expressing plasmid containing a gene therapy sequence in Escherichia coli DH1 bioreactor culture. We propose that we exploited a transient increase in specific plasmid content, first observed during the adaptation from batch to chemostat culture, by inducing a progressive down-regulation of growth rate during our fed batch culture.

Keywords
Gene therapy plasmid; Chemostat; Gene therapy process
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Fed batch culture with declining specific growth rate for high-yielding production of a plasmid containing a gene therapy sequence in Escherichia coli DH1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 1, 1 June 2006, Pages 47–50
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us