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Optimization of inulinase production by solid-state fermentation using sugarcane bagasse as substrate

Paper ID Volume ID Publish Year Pages File Format Full-Text
18751 42741 2006 4 PDF Available
Title
Optimization of inulinase production by solid-state fermentation using sugarcane bagasse as substrate
Abstract

The production of enzymes by bioprocesses is a good alternative to add value to agroindustry residues. Sugarcane bagasse is an abundant by-product of sugar industry and was tested as support and carbon source for production of inulinase (2,1-β-d-fructanohydrolase, E.C. 3.2.1.7) from Kluyveromyces marxianus NRRL Y-7571 by solid-state fermentation. Corn steep liquor was used as nitrogen supplement. Factorial design and response surface analysis were carried out to evaluate the effects of temperature (30.4–41.6 °C) and corn steep liquor (13–27.1%, w/v) on the production of inulinase. Optimum fermentation conditions were found to be: 36 °C and 20 wt.% of corn steep liquor. Under optimized conditions, the extra-cellular enzyme concentration reached 391.9 U/g of dry fermented bagasse.

Keywords
Inulinase; Optimization; Solid-state fermentation; Sugarcane; Bagasse
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 1, 1 June 2006, Pages 56–59
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us