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Purification and characterization of a novel cellooligosaccharide oxidase from rice pathogen Sarocladium oryzae

Paper ID Volume ID Publish Year Pages File Format Full-Text
18755 42741 2006 7 PDF Available
Title
Purification and characterization of a novel cellooligosaccharide oxidase from rice pathogen Sarocladium oryzae
Abstract

Sugar-oxidizing enzymes are widely detected in many fungal species. In this paper, a new cellooligosaccharide oxidase (COOX) was purified to homogeneity from wheat bran culture of a soil-isolated rice pathogen fungus strain, Sarocladium oryzae F137. This new sugar oxidase was composed of a single polypeptide chain with a molecular mass of 55 kDa and isoelectric point of 4.8–4.9. This enzyme contained 1 mol of FAD per mole of enzyme but no heme domain. This enzyme selectively bound to cellulose, but not to starch, chitin, or xylan. This enzyme oxidized oligosaccharides with reducing-end glucosyl residues linked by an β-1,4 glucosidic bond, such as lactose, cellobiose, and cellooligosaccharids. No significant substrate inhibition was observed at high concentrations of lactose, cellobiose, and maltose. COOX was used to produce a natural antioxidant, lactobionic acid, in a batch type reaction. The enzyme showed a preference for the two-electron acceptor 2,6-dichlorophenol-indophenol over the one-electron acceptors cytochrome c or ferricyanide. COOX did not contain a heme domain and used only two-electron acceptor. This feature could distinguish between the extracellular hemoflavoenzyme, cellobiose dehydrogenase, which is produced by a number of wood-degrading and phytopathogenic fungi.

Keywords
Cellooligosaccharide oxidase; Purification; Characterization; Solid-state fermentation
First Page Preview
Purification and characterization of a novel cellooligosaccharide oxidase from rice pathogen Sarocladium oryzae
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 1, 1 June 2006, Pages 85–91
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering