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Immobilization of thermostable trehalose synthase for the production of trehalose

Paper ID Volume ID Publish Year Pages File Format Full-Text
18759 42741 2006 6 PDF Available
Title
Immobilization of thermostable trehalose synthase for the production of trehalose
Abstract

Screening of several immobilization carriers for trehalose synthase (TSase) from Thermus caldophilus GK24 has been performed for the efficient production of trehalose from maltose. This is the first report on immobilization of recombinant TSase for the production of trehalose. Taking account of yields of trehalose produced, Eupergit C250L was selected as a carrier in this work. The immobilization capacity reached 11 units/g-supports (92% of immobilization yield) when the conditions were as follows: coupling time of 14 h at 25 °C in 40 mM potassium phosphate buffer (pH 7.0) and enzyme loading of 12 units/g-supports. The optimum pH was not affected by immobilization, but optimum temperature was shifted from 45 to 65 °C. Immobilized enzyme was stable at high temperature (70 °C) for 16 days, whereas free enzyme retained 13% of its original activity after 6 days of incubation. The immobilized TSase could be used in the repetitive manner more than 10 times in batch reaction. Using the continuous system (bed volume: 25 mL), a maximum yield of 42% trehalose has been reached from 50 g/L maltose when the flow rate was 0.25 ml/min.

Keywords
Eupergit C250L; Immobilization; Recombinant trehalose synthase; Thermus caldophilus; Packed bed; Trehalose
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Immobilization of thermostable trehalose synthase for the production of trehalose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 1, 1 June 2006, Pages 108–113
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us