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The effect of carbohydrates on α-amylase activity measurements

Paper ID Volume ID Publish Year Pages File Format Full-Text
18760 42741 2006 6 PDF Available
Title
The effect of carbohydrates on α-amylase activity measurements
Abstract

The Ceralpha method can be used for α-amylase activity measurements during the hydrolysis of starch at high substrate concentrations (>40 wt.%). However, the results are affected by the carbohydrates present in the samples. The effect of carbohydrates on the Ceralpha α-amylase activity measurements was measured over a broad concentration range. It was found that starch has the largest influence and glucose has the lowest influence on the Ceralpha assay procedure. These results were explained by considering substrate inhibition and substrate competition. A simple kinetic model was used to describe the observed phenomena quantitatively. This model was also used to estimate the Michaelis–Menten constant for a large number of substrates and it requires only a single experiment for each Km determination.

Keywords
α-Amylase activity assay; Ceralpha method; Carbohydrates; Kinetic model; Substrate inhibition; Substrate competition; Michaelis–Menten constant
First Page Preview
The effect of carbohydrates on α-amylase activity measurements
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 1, 1 June 2006, Pages 114–119
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering