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New method for the covalent immobilization of a xylanase by radical grafting of acrylamide on cellulose acetate membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
18762 42741 2006 6 PDF Available
Title
New method for the covalent immobilization of a xylanase by radical grafting of acrylamide on cellulose acetate membranes
Abstract

This work deals with a new grafting method of acrylamide on cellulose acetate powder and flat membranes, followed by activation with glutaraldehyde in order to provide supports for the enzyme immobilization. The grafting of acrylamide was proved by FTIR and by determination of nitrogen content of the cellulose acetate material. The influence of some polymerization parameters on the grafting degrees was checked. The grafting degrees of acrylamide, on cellulose acetate flat membranes, increased almost linearly with the initiator/membrane ratio, polymerization time and monomer/membrane ratio, whereas the activation time had a more complex influence. The immobilization of xylanase was successfully occurred on the acrylamide grafted CA membranes. The bonded enzyme has shown biocatalytic activity in beechwood xylan solution, after subsequent incubations at 50 °C, lasting 7 h altogether. The immobilized enzyme was more resistant to inactivation by heat than the soluble enzyme.

Keywords
Cellulose acetate membranes; Acrylamide; Grafting; Covalent immobilization; Xylanase
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New method for the covalent immobilization of a xylanase by radical grafting of acrylamide on cellulose acetate membranes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 1, 1 June 2006, Pages 125–130
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us