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Significant effect of NH4+ on cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris

Paper ID Volume ID Publish Year Pages File Format Full-Text
18774 42743 2006 8 PDF Available
Title
Significant effect of NH4+ on cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris
Abstract

Effects of nitrogen sources on cell growth and cordycepin production by submerged cultivation of Cordyceps militaris, a traditional Chinese medicinal mushroom, were investigated. In complex medium, peptone was identified as the best nitrogen source for cordycepin biosynthesis. A study using chemically defined medium indicated that NH4+ played an important role in cordycepin biosynthesis. To enhance cordycepin production, fed-batch culture with NH4+ feeding was conducted in complex medium with peptone. By optimizing the feeding time and feeding amount of NH4+, a maximal cordycepin concentration of 420.5 ± 15.1 mg/l was obtained, which was 70% higher than that in batch cultivation. The fed-batch cultivation process was also demonstrated in a 3.5 l stirred tank bioreactor and the cordycepin production was increased from 208.8 ± 7.6 mg/l in batch culture to 346.1 ± 3.6 mg/l in fed-batch culture. The information is useful for large-scale efficient production of cordycepin by the mushroom cultures.

Keywords
Medicinal mushroom; Submerged cultivation; Cordycepin; Cordyceps militaris; NH4+ effect; Fed-batch culture
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Significant effect of NH4+ on cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 3–4, 1 February 2006, Pages 343–350
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us