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Production of cellulases by Penicillium brasilianum IBT 20888—Effect of substrate on hydrolytic performance

Paper ID Volume ID Publish Year Pages File Format Full-Text
18779 42743 2006 10 PDF Available
Title
Production of cellulases by Penicillium brasilianum IBT 20888—Effect of substrate on hydrolytic performance
Abstract

The fungus Penicillium brasilianum IBT 20888 was cultivated on three different carbon sources to investigate the effect of the carbon source on the enzyme production. The carbon sources used were Sigmacell cellulose (SC), steam pretreated spruce (SPS) and a mixture of SC, oat spelts xylan and birchwood xylan (SCXX). Enzymatic assays and capillary electrophoresis revealed clear differences among three enzyme preparations produced—both in activity levels and in the distribution between enzymes within the same class. The hydrolysis efficiency of the resulting enzyme preparations was studied on SC and SPS. The three enzyme preparations performed equally well on SPS using an enzyme loading of 25 FPU (g cellulose)−1. The enzyme preparation produced on SCXX resulted in 15% less glucose released from SC compared to the two other enzyme preparations. A lower total cellobiohydrolase level could in part explain this difference. Adsorption studies revealed that three-fold more protein was adsorbed onto SPS compared to SC due to the presence of lignin in SPS. The binding onto lignin was demonstrated to be non-specific as most proteins in the enzyme preparations were adsorbed. It was also demonstrated by the use of CE that some degradation of the enzymes occurred during the hydrolysis.

Keywords
Capillary electrophoresis; Endoglucanases; Cellobiohydrolases; Hemicellulases; Adsorption
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Production of cellulases by Penicillium brasilianum IBT 20888—Effect of substrate on hydrolytic performance
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 3–4, 1 February 2006, Pages 381–390
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us