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Evaluation of cardosin A as a probe for limited proteolysis in non-aqueous environments—complex substrates hydrolysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
18783 42743 2006 7 PDF Available
Title
Evaluation of cardosin A as a probe for limited proteolysis in non-aqueous environments—complex substrates hydrolysis
Abstract

Cardosin A is an aspartic proteinase, like pepsin, purified from the cardoon, Cynara cardunculus L. and has been traditionally used in Portugal in the manufacture of traditional cheeses, due to its milk clotting activity. This enzyme is a fine example of a small number of plant aspartic proteinases that have been isolated, purified and characterised both in aqueous and in non-aqueous media.The present work deals with the effect of organic solvents on cardosin A specificity towards complex substrates such as κ-, β- and α-caseins and human type I collagen. At the same time fluorescence studies were carried out to correlate reaction rates with possible structural alterations induced by the organic solvents on the substrates. Results show a strong dependence of the velocity of hydrolysis on the medium composition and additionally, it is shown that none of the organic solvents used in this work, induced alterations of cardosin A specificity. Furthermore, it is shown that cardosin A in the presence of organic solvents can be used as a catalyst in order to obtain reaction intermediary products that are, otherwise, rapidly consumed in aqueous buffer.

Keywords
Aspartic proteinases; Caseins; Collagen; Organic solvents; Specificity; Cardosin A
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Evaluation of cardosin A as a probe for limited proteolysis in non-aqueous environments—complex substrates hydrolysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 3–4, 1 February 2006, Pages 415–421
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us