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Characterization of hemicellulases and cellulases produced by Ceriporiopsis subvermispora grown on wood under biopulping conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
18786 42743 2006 7 PDF Available
Title
Characterization of hemicellulases and cellulases produced by Ceriporiopsis subvermispora grown on wood under biopulping conditions
Abstract

Production of cellulases and hemicellulases by Ceriporiopsis subvermispora cultured on wood chips of Eucalyptus grandis and Pinus taeda was studied. Xylanase, endo-1,4 β-glucanase, mannanase, β-glucosidase and β-xylosidase were detected in enzymatic extracts obtained after 30 days of cultivation. P. taeda gave rise to higher enzyme production compared to E. grandis. Biochemical characteristics of cellulases and hemicellulases produced in both wood species were almost the same. The optimum pH for the enzymes was between 4.0 and 5.0 and the optimum temperature was 60 °C. Thermal stability of the enzymes at pH 5.0 was appreciable up to 50 °C. The profile of proteins contained in extracts recovered from both biotreated wood species showed a similar number of bands during SDS-PAGE electrophoresis. Five bands with molecular weight ranging from 50 to 85 kDa and two bands with high molecular weight (100–200 kDa) were observed. Activity staining for xylanases indicated two enzymes with molecular weight of 80 and 33 kDa.

Keywords
Ceriporiopsis subvermispora; Cellulases; Electrophoresis; Solid state cultivation; Xylanases
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Characterization of hemicellulases and cellulases produced by Ceriporiopsis subvermispora grown on wood under biopulping conditions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 3–4, 1 February 2006, Pages 436–442
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us