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Purification and catalytic properties of novel enantioselective lipase from Acinetobacter sp. ES-1 for hydrolysis of (S)-ketoprofen ethyl ester

Paper ID Volume ID Publish Year Pages File Format Full-Text
18787 42743 2006 6 PDF Available
Title
Purification and catalytic properties of novel enantioselective lipase from Acinetobacter sp. ES-1 for hydrolysis of (S)-ketoprofen ethyl ester
Abstract

A novel enantioselective lipase from Acinetobacter sp. ES-1 exhibiting an excellent enantioselectivity for (S)-ketoprofen ethyl ester was purified to homogeneity using hydrophobic interaction chromatography. The molecular weight and pI value of the purified enantioselective lipase were 32 kDa and 6.2, respectively, and the optimal temperature and pH were 40 °C and 7.0. The activity was enhanced 1.5-fold in the presence of Ca2+ ions, and showed the substrate specificity preferably for a medium-chain length, such as triacylglycerides and p-nitrophenyl esters. The N-terminal amino acid residue was A–V–R–S–I–D–G–L–A–I–N–Q–S–A, and no homology was found among other known lipases, indicating a novel type enzyme. The enantioselective hydrolysis of (R,S)-ketoprofen ethyl ester to optically pure (S)-ketoprofen was carried out in the aqueous phase reaction system achieving an high enantiomeric excess of 0.99 and conversion yield of 0.49 after 72 h.

Keywords
Lipase; Acinetobacter sp. ES-1; Enantioselectivity; (R,S)-Ketoprofen ethyl ester; (S)-Ketoprofen; Catalytic properties
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Purification and catalytic properties of novel enantioselective lipase from Acinetobacter sp. ES-1 for hydrolysis of (S)-ketoprofen ethyl ester
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 3–4, 1 February 2006, Pages 443–448
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us