fulltext.study @t Gmail

Immobilization procedures for the development of a biosensor for determination of hydroquinone using chitosan and gilo (Solanum gilo)

Paper ID Volume ID Publish Year Pages File Format Full-Text
18788 42743 2006 8 PDF Available
Title
Immobilization procedures for the development of a biosensor for determination of hydroquinone using chitosan and gilo (Solanum gilo)
Abstract

A novel reagentless biosensor for the square wave voltammetric determination of hydroquinone was developed by immobilization of gilo (Solanum gilo) crude extract in the chitosan biopolymer. The gilo tissue was obtained as a source of peroxidase and immobilized on a chitosan matrix using four different methods: physical adsorption, carbodiimide, glutaraldehyde and carbodiimide/glutaraldehyde. The highest biosensor performance was obtained after the immobilization of peroxidase in chitosan by activation of hydroxyl groups with carbodiimide and cross-linking with glutaraldehyde, which was incorporated in a carbon paste electrode. In the presence of hydrogen peroxide this enzyme catalyses the oxidation of hydroquinone to quinone and the silver/silver chloride (3.0 M KCl). The recovery of hydroquinone from the samples ranged from 95.0 to 105% and a rectilinear analytical curve for hydroquinone concentration from 0.25 to 5.5 mM (r = 0.9996) was obtained. The detection limit was 0.002 mM and the relative standard deviation was lower than 1.0% for 0.3 mM hydroquinone in 0.1 M phosphate buffer solution at pH 7.0 (n = 8). The lifetime of this biosensor was 6 months (at least 300 determinations).

Keywords
Chitosan; Gilo (Solanum gilo); Biosensor; Crude extract; Hydroquinone
First Page Preview
Immobilization procedures for the development of a biosensor for determination of hydroquinone using chitosan and gilo (Solanum gilo)
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 3–4, 1 February 2006, Pages 449–456
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us