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Optimization of the overexpression of glutamate mutase S component under the control of T7 system by using lactose and IPTG as the inducers

Paper ID Volume ID Publish Year Pages File Format Full-Text
18790 42743 2006 5 PDF Available
Title
Optimization of the overexpression of glutamate mutase S component under the control of T7 system by using lactose and IPTG as the inducers
Abstract

The bacteriophage T7 expression system is one of the most popular methods used to produce recombinant proteins in prokaryotic cells. IPTG is generally used as the inducer. Without the reconstruction of expression vector or the use of different expression system, a novel induction strategy to enhance the level of protein expression is reported in this study. Our results show that the yield of purified glutamate mutase S component (MutS) protein is increased three-fold by reducing the induction temperature to 20 °C and using 0.2% lactose and 50 mg/L IPTG simultaneously as inducers. Similar results are also observed in the production of other recombinant proteins. This strategy proved to be more efficient and cheaper for high-level expression of a foreign gene under the control of the T7 expression system in Escherichia coli, making it suitable for laboratory-scale preparation of recombinant proteins.

Keywords
T7 system; Lactose; Glutamate mutase S component; Expression
First Page Preview
Optimization of the overexpression of glutamate mutase S component under the control of T7 system by using lactose and IPTG as the inducers
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 3–4, 1 February 2006, Pages 465–469
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering