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Direct immobilization of peroxidase on DEAE cellulose from ammonium sulphate fractionated proteins of bitter gourd (Momordica charantia)

Paper ID Volume ID Publish Year Pages File Format Full-Text
18791 42743 2006 8 PDF Available
Title
Direct immobilization of peroxidase on DEAE cellulose from ammonium sulphate fractionated proteins of bitter gourd (Momordica charantia)
Abstract

The direct immobilization of peroxidases on DEAE cellulose from ammonium sulphate fractionated proteins of bitter gourd has been investigated. The activated DEAE cellulose was quite effective in high yield immobilization of peroxidases from bitter gourd and it could bind nearly 590 enzyme units per g of the matrix. Bitter gourd peroxidase immobilized on this anion exchanger showed very high effectiveness factor ‘η’ as 0.95. BGP bound very strongly to the DEAE cellulose, as it did not detach even in the presence of 0.5 M NaCl. Immobilized bitter gourd peroxidase preparation was more stable to the denaturation induced by pH, heat, urea, proteolytic enzyme, detergents (Surf Excel and Rin powder), Triton X 100 and water-miscible organic solvents (dioxane, dimethyl sulphoxide and n-propanol). Peroxidase adsorbed on the matrix exhibited very high resistance to proteolysis mediated by the trypsin treatment. DEAE cellulose bound bitter gourd peroxidase lost 45% of its initial activity after treatment with 2.5 mg trypsin per ml of incubation mixture for 1 h at 37 °C while the soluble enzyme lost nearly 65% of the initial activity under similar incubation conditions.

Keywords
BGP, bitter gourd peroxidase; DEAE, diethyl aminoethyl; DMSO, dimethyl sulphoxideAdsorption; Direct; DEAE cellulose; Bitter gourd; Immobilization; Peroxidase; Stabilization; n-Propanol
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Direct immobilization of peroxidase on DEAE cellulose from ammonium sulphate fractionated proteins of bitter gourd (Momordica charantia)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 38, Issues 3–4, 1 February 2006, Pages 470–477
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us