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Cultivation of Pichia capsulata as a whole-cell biocatalyst with NADH-dependent alcohol dehydrogenase activity for R-1-phenylethanol production

Paper ID Volume ID Publish Year Pages File Format Full-Text
18828 43035 2015 7 PDF Available
Title
Cultivation of Pichia capsulata as a whole-cell biocatalyst with NADH-dependent alcohol dehydrogenase activity for R-1-phenylethanol production
Abstract

•Cultivation process for the production of alcohol dehydrogenase was developed.•NADH-dependent enzyme from P. capsulata converted acetophenone to R-1-phenylethanol.•Optimal conditions for the production of whole-cell biocatalyst were found.

The aim of this work was to develop a cultivation process for the production of NADH-dependent alcohol dehydrogenase catalyzing a less common reduction of acetophenone to R-1-phenylethanol, an important compound for fragrance and flavor industry. Screening of 23 yeast strains showed that Pichia capsulata ATCC 16753 cells possess high catalytic activity for acetophenone reduction to R-1-phenylethanol. This high alcohol dehydrogenase activity was observed not only for whole cells but also for a raw enzyme solution using NADH as the cofactor. R-1-phenylethanol was produced in enantiomeric excess values of over 99% for the raw enzyme solution and of over 90% for the whole-cell biocatalyst cultivated at optimal conditions. The key factors for achieving high yield and selectivity were: proper cultivation time, glucose concentration and dissolved oxygen control.

Keywords
Alcohol dehydrogenase production; Bioreactor cultivation; Chiral reduction; Pichia capsulata; R-1-phenylethanol; Whole-cell biocatalysis
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Cultivation of Pichia capsulata as a whole-cell biocatalyst with NADH-dependent alcohol dehydrogenase activity for R-1-phenylethanol production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 96, October 2015, Pages 126–132
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us