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Simultaneous liquefaction, saccharification and fermentation at very high gravity of rice at pilot scale for potable ethanol production and distillers dried grains composition

Paper ID Volume ID Publish Year Pages File Format Full-Text
18860 43036 2016 7 PDF Available
Title
Simultaneous liquefaction, saccharification and fermentation at very high gravity of rice at pilot scale for potable ethanol production and distillers dried grains composition
Abstract

•We studied a very high gravity (311.5 g/l) process to produce ethanol from rice.•We reached 17.6% v/v ethanol (corresponding to a yield of 86.3%) at lab scale.•We reached 17.0% v/v (corresponding to a yield of 83.2%) at pilot scale (25 l).•Rice-based distillers dried grains with high protein content (47.5%) were obtained.

In this work, a simultaneous liquefaction, saccharification, and fermentation (SLSF) process at very high gravity (VHG) of broken rice for potable ethanol production was developed at pilot scale. The SLSF–VHG process was performed in a unique fermenter, at 30 °C. Rice flour (RF) was dissolved in tap water to reach 311.5 g/l dry matter and then the mixture was simultaneousely liquefied, saccharified, and fermented. Thanks to the addition of a raw starch hydrolyzing enzyme containing a mixture of alpha-amylase and gluco-amylase (Stargen 002 at 991.8 GAU/kg RF), gluco-amylase (Amigase Mega L at 0.035% w/w), protease (Fermgen at 600 SAPU/kg RF), active dry yeast Saccharomyces cerevisiae (Red Ethanol at 3.5 × 107 cells/ml), KH2PO4 (4.8 mM), and urea (16.0 mM). Under these conditions, the SLSF–VHG process finished after 120 h and achieved an ethanol content of 17.6% v/v corresponding to 86.3% of the theoretical ethanol yield. We scaled up this SLSF process at very high gravity at pilot scale (25 l) and achieved an ethanol content of 17.0% v/v corresponding to a yield of 83.2% of the theoretical ethanol yield. Rice-based distillers dried grains (DDG) was produced from the whole stillage of SLSF process at very high gravity by being plate-filtered and dried. The obtained DDG had high contents of crude protein (47.5%) and fibers (15.8%). Our results suggest, the SLSF under VHG condition of broken rice as well as the recovery of protein-rich DDG could have a great potential for the ethanol and animal feeding industry in Vietnam.

Keywords
Simultaneous liquefaction; Saccharification and Fermentation (SLSF); Very high gravity (VHG); Rice; Potable ethanol; Distillers dried grains
First Page Preview
Simultaneous liquefaction, saccharification and fermentation at very high gravity of rice at pilot scale for potable ethanol production and distillers dried grains composition
Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 98, April 2016, Pages 79–85
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering