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Bioprocess challenges to the isolation and purification of bioactive peptides

Paper ID Volume ID Publish Year Pages File Format Full-Text
18877 43036 2016 13 PDF Available
Title
Bioprocess challenges to the isolation and purification of bioactive peptides
Abstract

•Bioactive peptides (BPs) trigger physiological responses that can promote health.•BPs have significant potential as active components in the formulation of functional foods and peptide-based therapeutics.•Scale-up manufacturing of BPs is hampered by downstream bioprocessing challenges relating to biomolecular purification.•‘-Omics’ techniques and chromatographic purification using monolithic adsorbents offer prospects to overcome these challenges.

Food protein-derived bioactive peptides (BPs) have been reported to trigger certain physiological responses in the body, thereby influencing health positively. These peptides have attracted high research and consumer interests due to their huge potential of use in functional foods and other dietary interventions of disease control and health promotion. However, successful product development is limited by the fact that current manufacturing processes are either difficult to scale up, high in cost, or have the potential to affect the structure-activity properties of these peptides. To overcome these challenges, we have proposed in this review, the use of an integrated ‘-omics’ approach comprising in silico analysis and ‘-omics’ techniques (such as peptidomics) to respectively forecast and validate the biological and physico-chemical properties of the peptides. This information is then used for the rational design of suitable purification steps for peptides of interest. Downstream purification could also be undertaken by liquid chromatography using monolithic adsorbents physico-chemically engineered (using results of in silico analysis) for rapid isolation of peptides. By coupling the high throughput and predictive capability of ‘-omics’ to the enhanced convective hydrodynamics of monolithic columns, it becomes feasible, even at preparative scale, to produce BPs that meet the requirements of high purity, potency, and cost-effectiveness.

Keywords
ACE, angiotensin-I-converting enzyme; DH, degree of hydrolysis; EMF, electromembrane filtration; MC, membrane chromatography; QSAR, quantitative structure–activity relationship; QSPR, quantitative structure–property relationship; HILIC, hydrophilic intera
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 98, April 2016, Pages 244–256
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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