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Evaluation of cross-linked enzyme aggregates of Lactobacillus cell-envelope proteinases, for protein degradation

Paper ID Volume ID Publish Year Pages File Format Full-Text
18897 43037 2015 11 PDF Available
Title
Evaluation of cross-linked enzyme aggregates of Lactobacillus cell-envelope proteinases, for protein degradation
Abstract

•Cell-envelope proteinases have been prepared as cross-linked enzyme aggregates.•Cross-linking conditions have been optimized to achieve high enzyme activity.•Cross-linking improves recyclability and stability of cell-envelope proteinases.•Proteolytic performance has been evaluates using several food proteins.

Enzymatic hydrolysis is a widely used approach to improve the functional, nutritional and physiological properties of food proteins. In this study, cross-linked enzyme aggregates (CLEAs) have been prepared from cell-envelope proteinases (CEPs) of Lactobacillus delbrueckii subsp. lactis 313 and their proteolytic properties have been evaluated using several food proteins. We have optimized cross-linking conditions including ammonium sulphate concentration, incubation temperatures, agitation speed, glutaraldehyde cross-linker concentration, reaction time and the addition of proteic feeders. Particularly, the presence of BSA improves retained activity of cross-linked CEP aggregates (CLCEPAs) from 21.5% to 40.9%. Blocking unreacted cross-linking groups on aggregates is important to enhance recyclability of CLCEPAs. CLCEPAs had attractive thermal stability at 50 °C and it showed enhanced catalytic activity over long-term storage after lyophilization. We have demonstrated that CLCEPAs has proteolytic properties on different food proteins including complex (chicken egg albumin, skimmed-milk protein), fractionated (bovine casein, whey protein isolate), and purified (bovine serum albumin) proteins. Being the first report of CLEAs from lactobacilli CEPs, this study demonstrates the feasibility of using LDL 313 CLCEPAs for degradation of various food proteins.

Keywords
CEPs, cell-envelope proteinases; CLEAs, cross-linked enzyme aggregates; CLCEPAs, cross-linked CEP aggregates; CLECs, cross-linked enzyme crystals; LDL 313, Lactobacillus delbrueckii subsp. lactis 313; CLCEPAs-BSA, cross-linked CEP aggregates prepared with
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Evaluation of cross-linked enzyme aggregates of Lactobacillus cell-envelope proteinases, for protein degradation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 94, April 2015, Pages 59–69
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us