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Enzymatic modification of sesame seed protein, sourced from waste resource for nutraceutical application

Paper ID Volume ID Publish Year Pages File Format Full-Text
18898 43037 2015 12 PDF Available
Title
Enzymatic modification of sesame seed protein, sourced from waste resource for nutraceutical application
Abstract

•Successful recovery of nutritionally rich protein concentrates from waste product.•Subsequent hydrolysis of the protein concentrate improved functional properties.•Ultrafiltration results in efficient yield of short peptides, evinced by MALDI-TOF.•Potent ACE-inhibitory activity of these short peptides confirmed its bioactivity.

The functional characteristics which include protein solubility at different pH, emulsifying and foaming properties, degree of hydrolysis, molecular weight distribution, antioxidant and ACE inhibitory activity of sesame protein hydrolysates prepared with pepsin, papain and alcalase enzymes were evaluated. The rate of degree of hydrolysis was found to reach maximum (25–30%) within the first time fragment i.e 10 min but 80% of hydrolysis was obtained in 120 min with alcalase. SDS-PAGE of hydrolysates with papain, pepsin and alcalase evinced bands of low molecular weight protein of 14.3 kDa and even lower for alcalase treatment of 120 min. Hydrolysates so formed were of improved functional properties as evident from emulsifying and foaming property. Hydrolysis with different proteases enhanced the protein solubility significantly at pH 7.0. Antioxidative assay revealed radical scavenging activity of the hydrolysates with papain hydrolysates showing maximum antioxidative efficacy. The ultra-filtered peptide fractions which showed comparable ACE inhibitory activity were sequenced by MALDI-TOF and matched to that of previously identified ACE inhibitory peptides. The results corroborate the ACE inhibitory effect of the peptides. Hence, these highly bioactive protein hydrolysates produced from waste sesame meals can be successfully employed in various functional food formulations.

Keywords
Sesame protein hydrolysates; Functional properties; Bioactive peptides; Antioxidant; Angiotensin converting enzyme (ACE) inhibitorWSM, whole sesame meal; DDSM, dehulled and deoiled sesame meal; SPC, sesame protein concentrate; PapH60, SPC hydrolysates by
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Enzymatic modification of sesame seed protein, sourced from waste resource for nutraceutical application
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 94, April 2015, Pages 70–81
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us