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Evaluation of sawdust hemicellulosic hydrolysate for bioproduction of xylitol by enzyme xylose reductase

Paper ID Volume ID Publish Year Pages File Format Full-Text
18899 43037 2015 8 PDF Available
Title
Evaluation of sawdust hemicellulosic hydrolysate for bioproduction of xylitol by enzyme xylose reductase
Abstract

•Meranti wood sawdust hemicellulosic hydrolysate can be a potential xylose source for enzymatic xylitol production.•Parameter design was conducted to select the effective range of process factors.•Xylitol obtained was 10.53 g/L which corresponds to a yield of 56%.•Results are validated against experimental literature data.

Sawdust hemicellulosic hydrolysate can be used as a promising xylose source for enzymatic conversion to xylitol, a specialty product. The aim of this study was to design various factors by observing their effects on xylitol production from hemicellulosic hydrolysate by xylose reductase (XR) enzyme. The one-factor-at-a-time (OFAT) strategy was adopted to determine the effective levels of process factors influencing xylitol yield. Enzymatic xylitol production was conducted in batch mode using different levels of reaction time (2–18 h), temperature (20–70 °C), pH (4.0–9.0), xylose concentration (9.4–37.6 g/L), NADPH concentration (1.17–5.32 g/L), enzyme concentration (2–6%), and agitation (50–150 rpm). Among the factors examined, time, temperature, pH, NADPH, and enzyme concentration significantly influenced the biosynthesis of xylitol with the suitable level of 10 h, 30 °C, 7.0, 3.66 g/L, and 3% (v/v), respectively. Xylitol yield obtained was 56% (w/w) under these observed optimum conditions. This is the first report on the bioproduction of xylitol from lignocellulosic substrate by XR.

Keywords
Biotransformation; Hemicellulosic hydrolysate; Parameter design; Xylitol; Xylose; Xylose reductase
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Evaluation of sawdust hemicellulosic hydrolysate for bioproduction of xylitol by enzyme xylose reductase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 94, April 2015, Pages 82–89
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us