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Effects of alkali treatment and subsequent acidic extraction on the properties of soybean soluble polysaccharides

Paper ID Volume ID Publish Year Pages File Format Full-Text
18914 43037 2015 9 PDF Available
Title
Effects of alkali treatment and subsequent acidic extraction on the properties of soybean soluble polysaccharides
Abstract

•We obtained SSPS with different DE values by alkali treatment condition.•The alkali treatment condition had prominent effects on properties of SSPS.•When SSPS had low DE, the absolute negative charges was big.•The lower DE was, the better emulsifying and stabilizing activities were.•We could produce SSPS of better functional properties.

Soybean soluble polysaccharides (SSPS) were extracted under acidic condition from the okara of producing soybean protein isolate. The methyl esterificated carboxylic group of galacturonic acid (GalA) in SSPS could be demethoxylated by alkali treatment when heated, which had effects on SSPS properties. In this study, okara was treated in six selected alkali conditions, and then SSPS were extracted under the acidic condition (115 °C, 100 min, pH 4.5). The obtained SSPS were determined to have degree of esterification (DE) of 49, 55, 60, 66, 74 and 83%, respectively. Violent alkali treatment produced SSPS with not only lower DE but also lower molecular weight (MW) compared to mild alkali treatment. MW was increased from 396 to 489 kDa with increasing DE. It was found that SSPS with lower DE possessed lower aqueous viscosity, more negative charges, better emulsifying activity in oil-in-water emulsion and stabilizing activity in acidic milk drink. When DE changed from 49 to 83%, aqueous viscosity (10% (w/w) SSPS), average particle size of fresh oil-in-water emulsion, and centrifugation precipitating rate (CPR) of acidic milk drink (pH 4.5; two-stage 30 MPa homogeneous pressure) were increased from 25.8 to 108.2 mPa s, 1.5 to 4.0 μm, and 0.20 to 0.47%, respectively; and zeta potential (pH 7) was decreased from 17 to 10 mV. These results suggested that SSPS obtained from different alkali treatment had different functional properties.

Keywords
Soybean soluble polysaccharide; Alkali treatment; Acidic extraction; Degree of esterification; Molecular weight; Functional properties
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Effects of alkali treatment and subsequent acidic extraction on the properties of soybean soluble polysaccharides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 94, April 2015, Pages 239–247
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us