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Recovery of a bioactive tripeptide from a crude hydrolysate using activated carbon

Paper ID Volume ID Publish Year Pages File Format Full-Text
18916 43037 2015 8 PDF Available
Title
Recovery of a bioactive tripeptide from a crude hydrolysate using activated carbon
Abstract

•Enrichment of a single bioactive peptide using activated carbon.•Adsorption process described for several adsorptive−desorptive cycles.•Gradient elution and peak fractionation during desorption lead to purity increase.•Guidelines for more suitable activated carbon characteristics have been deduced.

Separation and purification processes of neutraceuticals, such as bioactive peptides, are usually done in a multistep process that sometimes requires a final chromatographic step using expensive resins. Activated carbon is a promising economic alternative for the resins. We report here on the application of a hydrophobic interaction on a chromatographic column packed with particles of activated carbon to isolate a lactotripeptide from a crude hydrolysate. Consecutive adsorptive–desorptive cycles were used until exhaustion of the column. Liquid chromatography–mass spectrometry results showed an enrichment of the lactotripeptide isoleucine–proline–proline with a yield of up to 80% in the third cycle and a twofold increase in purity to up to 35%. Some guidelines are given for the competitive exhaustion of the adsorbent for process optimization in order to obtain higher purity and yield.

Keywords
Bioactive peptide enrichment; Hydrolysate; Adsorption; Activated carbon; Hydrophobic interaction chromatography; Gradient elution, LC
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Recovery of a bioactive tripeptide from a crude hydrolysate using activated carbon
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 94, April 2015, Pages 255–262
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us