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Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments

Paper ID Volume ID Publish Year Pages File Format Full-Text
18932 43037 2015 7 PDF Available
Title
Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments
Abstract

•Pre-enzymatic treatment combined with 70 °C treatment, increased fructan extraction.•High temperature affected extraction in ultrasonic processes.•Fructan degradation was minimized during enzymatic process.•Sonothermal and enzymatic pretreatment affected cell wall.

The effects of different extraction methods of water-soluble carbohydrates (WSC) from the sotol plant (Dasylirion leiophyllum) were investigated. Sotol fragments were extracted at 40 and 70 °C, under thermal treatment (T), pre-enzymatic thermal treatment (PET), sonothermal treatment (ST), and pre-enzymatic sonothermal treatment (PEST) conditions: fructose, glucose, sucrose, and fructans were analyzed by HPLC and the total water soluble carbohydrates was determined. At 70 °C, the highest WSC values (482 mg/gd.m.) were obtained, with a fructan proportion of 69%. Pre-enzymatic treatment at 70 °C resulted in a high WSC content with the highest fructans proportion (87%) and lowest contents of RS and sucrose. The effect of the interaction between ultrasound and enzymatic treatments was limited by the high-temperature effect (70 °C), thereby minimizing the extraction. Microscopy analyses showed cell-wall modifications with the ST and PET treatments, which caused an increase in the total soluble sugars. The combination of enzymatic and sonothermal treatments at 70 °C resulted in the extraction of fructans in a higher yield and with less degradation. This circumvents the need for traditional high-energy processes, which could be beneficial for the extraction of WSC such as fructans from sotol or other economically important plants.

Keywords
T, thermal treatment; PET, pre-enzymatic thermal treatment; ST, sonothermal treatment; PEST, pre-enzymatic sonothermal treatment; WSC, water soluble carbohydrates; RS, reducing sugars; RH, relative humidity; TEM, transmission electron microscopyDasylirion
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Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments
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Publisher
Database: Elsevier - ScienceDirect
Journal: Food and Bioproducts Processing - Volume 94, April 2015, Pages 398–404
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us